Levels of amino acids and glycated, oxidised, or nitrated proteins in culture media of chondrocytes cultivated in multi-layers and in the blood of guinea pigs or osteoarthritis patients were studied by mass spectrometry.
This study analysed 60 male, 3-week-old guinea pigs. At 4-weeks and subsequently 8-week intervals until week 36, 12 animals were sacrificed and evaluated for histological severity of knee osteoarthritis and cartilage vascularisation. Patients with early and advanced osteoarthritis and healthy subjects were recruited. Human chondrocytes cultured in multilayers were treated for 10 days with IL-1β. Amino acids and glycated, oxidised, and nitrated proteins were analysed in the serum of guinea pigs, osteoarthritis patients and in the culture medium conditioned by chondrocytes by stable isotopic dilution analysis liquid chromatography-tandem mass spectrometry.
The results showed that the severity of osteoarthritis increased progressively in guinea pigs wi...
please login to read the entire article:
You need to register to read the entire article, please do so now.
« The Role of bDMARDs in Psoriatic Arthritis Next Article
Promising Results Rituximab in Systemic Sclerosis, and Systemic Lupus Erythematosus Classification Criteria »
Table of Contents: EULAR 2018
Psoriasis and Psoriatic Arthritis
Osteoporosis and Osteoarthritis
Systemic Sclerosis and Systemic Lupus Erythematosus
Necessary cookies are absolutely essential for the website to function properly. This category only includes cookies that ensures basic functionalities and security features of the website. These cookies do not store any personal information.
Any cookies that may not be particularly necessary for the website to function and is used specifically to collect user personal data via analytics, ads, other embedded contents are termed as non-necessary cookies. It is mandatory to procure user consent prior to running these cookies on your website.