Home > Rheumatology > EULAR 2018 > Osteoporosis and Osteoarthritis > Glucosepane Is a New Biomarker for the Severity of Osteoarthritis

Glucosepane Is a New Biomarker for the Severity of Osteoarthritis

Conference
EULAR 2018

Levels of amino acids and glycated, oxidised, or nitrated proteins in culture media of chondrocytes cultivated in multi-layers and in the blood of guinea pigs or osteoarthritis patients were studied by mass spectrometry.

This study analysed 60 male, 3-week-old guinea pigs. At 4-weeks and subsequently 8-week intervals until week 36, 12 animals were sacrificed and evaluated for histological severity of knee osteoarthritis and cartilage vascularisation. Patients with early and advanced osteoarthritis and healthy subjects were recruited. Human chondrocytes cultured in multilayers were treated for 10 days with IL-1β. Amino acids and glycated, oxidised, and nitrated proteins were analysed in the serum of guinea pigs, osteoarthritis patients and in the culture medium conditioned by chondrocytes by stable isotopic dilution analysis liquid chromatography-tandem mass spectrometry.

The results showed that the severity of osteoarthritis increased progressively in guinea pigs wi...



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